Molecular, Cellular and Organism High Throughput Screening for Hazard Assessment

Leader: André Nel
The main goal of Theme 2 is to use high content screening (HCS) and high throughput screening (HTS) for Engineered Nanomaterials (ENMs) at cellular and organismal (zebrafish) levels to develop predictive toxicological paradigms, hazard ranking and SARs to guide nano EHS decision-making and ecological research (Themes 4 and 5). HTS of industrially important silica, Cu, metal oxide (MOx), rare earth oxides (REO) and III-V semiconductor as well as doped CuO and fumed silica (Theme 1) were used since February 2016 for cellular, bacterial, and zebrafish screening. HTS was also used to assist center-wide copper and carbonaceous material case studies.  The work demonstrates the use of nanomaterial libraries that can be used for the toxicological profiling of broad and specific nanomaterial categories in order to information that can be used by the center at large as well as 3rd parties for the performance of toxicological profiling that can assist regulatory activity.  The work reflects close cooperation between investigators in Theme 1, Theme 2, and Theme 4.